Associado - Artigos

Menstrual endometrial cells from women with endometriosis demonstrate increased adherence to peritoneal cells and increased expression of CD44 splice variants

Jason S. Griffith, M.D., Ya-Guang Liu, M.D., Rajeshwar R. Tekmal, Ph.D., Peter A. Binkley, B.S., Alan E. C. Holden, Ph.D., and Robert S. Schenken, M.D.

Department of Obstetrics and Gynecology, University of Texas Health Science Center at San Antonio, San Antonio, Texas

Objective: We previously demonstrated that adherence of endometrial epithelial (EECs) and stromal cells (ESCs) to peritoneal mesothelial cells (PMCs) is partly regulated by ESC/EEC CD44 interactions with PMC associated hyaluronan. CD44, a transmembrane glycoprotein and major ligand for hyaluronan, has numerous splice variants which may impact hyaluronan binding. Here, we assessed whether ESCs and EECs from women with endome- triosis demonstrate increased adherence to PMCs and examined CD44 splice variants’ potential role in this process.
Design: In vitro study. Setting: Academic medical center. Patient(s): Fertility patients with and without endometriosis. Intervention(s): Menstrual endometrium was collected from women with and without endometriosis confirmed surgically. The adherence of ESC/EECs to PMCs was measured. The ESC/EEC CD44 splice variants were as- sessed using dot-blot analysis. Result(s): TheESCsandEECsfromwomenwithendometriosisdemonstratedincreasedadherencetoPMCs.The predominant CD44 splice variants expressed by ESCs and EECs from women with and without endometriosis were v3, v6, v7, v8, v9, and v10. The ESCs and EECs from women with endometriosis were more likely to express v6, v7, v8, and v9. Conclusion(s): Increasedeutopicendometrial-PMCadherenceandCD44splicevariantexpressionmaycontribute to the histogenesis of endometriotic lesions. Elucidation of factors controlling this expression may lead to novel endometriosis therapies. (Fertil SterilÒ 2010;93:1745–9. Ó2010 by American Society for Reproductive Medicine.)
Key Words: Endometriosis, endometrium, CD44, hyaluronan, splice variant, adherence

Clique aqui para download do artigo

Comentários feitos por associados

Clique aqui e envie sua pergunta ou comentário.